Meningitis is inflammation of the membranes (meninges) that surround the brain and spinal cord.
Meningitis can be caused by infectious agents like bacteria or viruses, a fungus, or parasites. Some cases of meningitis can be noninfectious in origin.
Headache,high grade fever, and stiff neck are the most common symptoms of meningitis. Confusion or lethargy can also be present.
Physicians usually make the diagnosis by performing a lumbar puncture (spinal tap) and evaluating the cerebral spinal fluid (CSF).The CSF is collected in a sterile container which is leakproof and contamination proof. The sample is sent to laboratory for isolation of the causative organism from the CSF. The procedure of Accession and Extraction of genetic material is followed meticulously. The extracted genetic material is taken to the master mixing room and reagents added.The master mix is the taken to the PCR machine.The process of amplification of the genetic material happens here and the depending on the genetic material presence the organism is isolated.
The PCR is a gold standard in identifying organisms.